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Which of the following statements about the light reactions in photosynthetic plants is false?


A) A membrane-bound ATPase couples ATP synthesis to electron transfer.
B) No CO2 is fixed in the light reactions.
C) The ultimate electron acceptor is O2.
D) The ultimate source of electrons for the process is H2O.
E) There are two distinct photosystems,linked together by an electron transfer chain.

F) C) and D)
G) None of the above

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Oxidative phosphorylation and photophosphorylation share all of the following except:


A) chlorophyll.
B) involvement of cytochromes.
C) participation of quinones.
D) proton pumping across a membrane to create electrochemical potential.
E) use of iron-sulfur proteins.

F) B) and E)
G) D) and E)

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During electron transfer through the mitochondrial respiratory chain,the overall reaction is: NADH + 1/2 O2 + H+ \rightarrow NAD+ + H2O.The difference in reduction potentials for the two half-reactions ( Δ\Delta E'°)is +1.14 V.Show how you would calculate the standard free-energy change, Δ\Delta G'°,for the reaction as written above.(The Faraday constant, During electron transfer through the mitochondrial respiratory chain,the overall reaction is: NADH + 1/2 O<sub>2</sub> + H<sup>+</sup>  \rightarrow  NAD<sup>+</sup> + H<sub>2</sub>O.The difference in reduction potentials for the two half-reactions ( \Delta E'°)is +1.14 V.Show how you would calculate the standard free-energy change, \Delta G'°,for the reaction as written above.(The Faraday constant, ,is 96.48 kJ/V·mol. ) ,is 96.48 kJ/V·mol. )

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blured imageG'° = -nblured imageblured imageE'° = (-2)(9...

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Which of the following electron carriers is not able to transfer one electron at a time?


A) NADH
B) FMN
C) FAD
D) Ubiquinone
E) Heme

F) A) and B)
G) A) and C)

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The skunk cabbage (Symphocarpus foetidus)can maintain a temperature of 10-25 °C higher than the temperature of the surrounding air.Suggest a mechanism for this.

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One possible heat-generating mechanism i...

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Although molecular oxygen (O2)does not participate directly in any of the reactions of the citric acid cycle,the cycle operates only when O2 is present.Explain this observation.

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The citric acid cycle produces NADH,whic...

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Mutations in mitochondrial genes play a role in each of the following diseases except:


A) adult onset diabetes.
B) cystic fibrosis.
C) hypertrophic cardiomyopathy.
D) Leber's hereditary optic neuropathy.
E) myoclonic epilepsy.

F) A) and B)
G) None of the above

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The rate of oxidative phosphorylation in mitochondria is controlled primarily by:


A) feedback inhibition by CO2.
B) the availability of NADH from the TCA cycle.
C) the concentration of citrate (or) the glycerol-3-phosphate shuttle.
D) the mass-action ratio of the ATD-ADP system.
E) the presence of thermogenin.

F) A) and B)
G) D) and E)

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Which of the following is correct concerning the mitochondrial ATP synthase?


A) It can synthesize ATP after it is extracted from broken mitochondria.
B) It catalyzes the formation of ATP even though the reaction has a large positive Δ\Delta G'°.
C) It consists of Fo and F1 subunits,which are transmembrane (integral) polypeptides.
D) It is actually an ATPase and only catalyzes the hydrolysis of ATP.
E) When it catalyzes the ATP synthesis reaction,the Δ\Delta G'° is actually close to zero.

F) None of the above
G) A) and D)

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For each of the following explain whether they are transported into or out of the mitochondrion and comment briefly on the mechanism for each: (1)NADH (2)Inorganic phosphate (3)ADP

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NADH produced by glycolysis is indirectl...

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Discuss three lines of evidence that support the theory that mitochondria evolved from endosymbiontic bacteria.

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1)Modern prokaryotes have respiratory ch...

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The standard reduction potential for ubiquinone (Q or coenzyme Q)is 0.045 V,and the standard reduction potential (E'°)for FAD is -0.219 V.Using these values,show that the oxidation of FADH2 by ubiquinone theoretically liberates enough energy to drive the synthesis of ATP.The Faraday constant, The standard reduction potential for ubiquinone (Q or coenzyme Q)is 0.045 V,and the standard reduction potential (E'°)for FAD is -0.219 V.Using these values,show that the oxidation of FADH<sub>2</sub> by ubiquinone theoretically liberates enough energy to drive the synthesis of ATP.The Faraday constant, ,is 96.48 kJ/V·mol. \Delta G'° for ATP synthesis is +30.5 kJ/mol. ,is 96.48 kJ/V·mol. Δ\Delta G'° for ATP synthesis is +30.5 kJ/mol.

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blured imageE'° = E'°(oxidant)- E'°(reductant)= 0.04...

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Which of the following is not found in modern cyanobacteria?


A) PSI
B) PSII
C) NADH dehydrogenase complex I
D) Oxygen-reducing complex IV
E) Mitochondria

F) A) and C)
G) A) and B)

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Which one of the following is true about reaction centers?


A) Cyanobacteria and plants have two reaction centers arranged in tandem.
B) Cyanobacteria contain a single reaction center of the Fe-S type.
C) Green sulfur bacteria have two reaction centers arranged in tandem.
D) Plant photosystems have a single reaction center of the pheophytin-quinone type.
E) Purple bacteria contain a single reaction center of the Fe-S type.

F) A) and D)
G) D) and E)

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Describe and explain the two different ratios that affect the rate of respiration in mitochondria.What is accomplished by these control mechanisms?

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The acceptor control ratio describes how...

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Upon the addition of 2,4-dinitrophenol (DNP) to a suspension of mitochondria carrying out oxidative phosphorylation linked to the oxidation of malate,all of the following occur except:


A) oxygen consumption decreases.
B) oxygen consumption increases.
C) the P/O ratio drops from a value of approximately 2.5 to 0.
D) the proton gradient dissipates.
E) the rate of transport of electrons from NADH to O2 becomes maximal.

F) A) and B)
G) A) and C)

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Which of the following is not controlled by hypoxia-inducible factor (HIF-1) ?


A) Glucose transport
B) Glycolysis
C) Citric acid cycle
D) Compex I of the respiratory chain
E) Complex IV of the respiratory chain

F) A) and C)
G) B) and D)

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In the reoxidation of QH2 by purified ubiquinone-cytochrome c reductase (Complex III) from heart muscle,the overall stoichiometry of the reaction requires 2 mol of cytochrome c per mole of QH2 because:


A) cytochrome c is a one-electron acceptor,whereas QH2 is a two-electron donor.
B) cytochrome c is a two-electron acceptor,whereas QH2 is a one-electron donor.
C) cytochrome c is water soluble and operates between the inner and outer mitochondrial membranes
D) heart muscle has a high rate of oxidative metabolism,and therefore requires twice as much cytochrome c as QH2 for electron transfer to proceed normally.
E) two molecules of cytochrome c must first combine physically before they are catalytically active.

F) B) and C)
G) D) and E)

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During photophosphorylation in plants,electrons flow through a series of carriers in the chloroplast.What is the ultimate donor of electrons,and what is the ultimate acceptor? What provides the energy to move those electrons?

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The ultimate donor is H2O,and t...

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Place the following electron-carriers into the proper order as found in plant chloroplasts. 1) Cytochrome b6f complex 2) P680 3) P700 4) Plastocyanin 5) NADPH


A) 2,1,3,4,5
B) 2,4,3,1,5
C) 3,1,4,2,5
D) 2,1,4,3,5
E) 3,4,2,1,5

F) C) and D)
G) A) and B)

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